Llantwit Major have been drawn at home to Afan Lido in the first round of the Nathaniel MG Cup.
It will mark Karl Lewis' first game as manager in three years following his recent return to the Cymru South club.
The winners will get home advantage against either Swansea City or Carmarthen Town in the second round.
Llantwit will be aiming to go better their second round exit at home to Cymru Premier club Cardiff Met in last season's competition.
Swansea are one of just two wildcard entries in the league cup - with Cardiff City entertaining Ammanford in the first round.
The draw, announced on Wednesday night, also includes a tie between two newly-promoted clubs - Ynyshir Albions and Cardiff Draconians.
Barry Town United enter the fray in the second round, starting with a home game against Aberystwyth Town, who were relegated from the Cymru Premier after 33 years as one of the league's founder members.
Barry, under then-manager Steve Jenkins (now the club's head of football), reached the semi-finals last season before going out to eventual winners The New Saints, who went onto beat Aberystwyth.
The first round ties will be played on the weekend of Friday 18th - Sunday 20th July - with the second round being played on the weekend of Friday 1st - Sunday 3rd July.
To watch the draw, visit the FAW's RedWall+ channel.
Nathaniel MG Cup southern section
First round
Cardiff City v Ammanford
Newport City v Baglan Dragons
Treowen Stars v Cambrian United
Swansea City v Carmarthen Town
Pontypridd United v Cwmbran Celtic
Ynyshir Albions v Cardiff Draconians
Caerau Ely v Trefelin
Llantwit Major v Afan Lido
Second round
Cardiff City / Ammanford v Treowen Stars / Cambrian United
Barry Town United v Aberystwyth Town
Briton Ferry Llansawel v Trethomas Bluebirds
Llantwit Major / Afan Lido v Swansea City / Carmarthen Town
Ynyshir Albions / Cardiff Draconians v Llanelli Town
Haverfordwest County v Newport City / Baglan Dragons
Caerau Ely / Trefelin v Penybont
Cardiff Met v Pontypridd United / Cwmbran Celtic
Llantwit and Barry await league cup draws
Barry sign ex-TNS defender Davies
Home win in inaugural Mary Thomas Trophy
Dimond says final farewell to Bombers
'True hero' David retires as Barry Town secretary
Ingle named in Women's Euros squad
Strongman Jacob puts new gym to the test
Bombers reunited at Reservoir Field
Darcey wins first European tour title
Record-breaking Barry Island 10K returns
Llantwit secure pitch maintenance funding
Llantwit announce pre-season fixtures
Barry tennis club plays pickleball
Golden Glove for Barry's Molly
Hulbert makes team of the season
Looking back and looking ahead at Bombers' awards night
