Flankers Alex Callender and Kate Williams will co-captain a side, with three changes, one positional, for the Test match at the Cardiff Arms Park on Saturday 26th September (KO 3pm)
Second row Georgia Evans will win her 50th cap for her country, while Carys Cox returns from injury and is selected on the wing.
Evans, who made her Wales debut against Spain in 2019, is part of an unchanged Welsh pack that beat South Africa 50-19 in the Vodafone Wales WXV Series opener.
Sisilia Tuipulotu, who scored two tries against South Africa, packs down with hooker Kelsey Jones and prop Maisie Davies in the Wales front row.
Lock Evans packs down with Branwen Metcalfe in the Welsh engine room, with skippers Callender and Williams in the backrow with Number 8 Bryonie King.
Scrum half Keira Bevan and fly half Lleucu George are selected at half back, while Cox is drafted in on the wing.
Centres Courtney Keight and Freya Bell are in midfield, while wing Cox joins wing Amy Williams and full back Lisa Neumann in the back three.
Scrum half Seren Lockwood was ruled out of selection due to HIA but will be available for the two WXV Tests in Japan.
Sean Lynn, Wales Head coach, said:
"We produced a performance against South Africa that demonstrated that we have moved the dial and all the hard work we put in during pre-season was there for all to see.
"We know we have to back that performance up against a fit, fast and athletic USA Eagles side which will turn up full of confidence after their win against Ireland in Dublin.
"USA are ranked six in the world rankings and five places above us, but we are excited by the challenge and want to build on the win against South Africa.
"The Welsh public saw the style of play we are committed to building, and we play for them and want to make them proud. To have their noisy support and backing at Cardiff Arms Park on Saturday can only inspire this Wales squad.
"Georgia Evans will win her 50th cap for her country and deserves the accolade, ‘G’ produced a trademark performance last time out and her work rate and commitment to her nation is an inspiration to all of us.
"We welcome Carys Cox back after her return from injury and Coxy knows she has to perform with competition for places being part of the DNA we are building in the squad."
Wales team (v USA)
15 Lisa Neumann (Leicester Tigers) 53 caps, 40 points
14 Carys Cox (Ealing Trailfinders) 26 caps, 30 points
13 Courtney Keight (Sale Sharks) 34 caps, ten points
12 Freya Bell (Gwalia Lightning) Three caps, 10points
11 Amy Williams (Brython Thunder) One
10 Lleucu George (Gloucester-Hartpury) 41 caps, 15 points
9 Keira Bevan (Bristol Bears) 84 caps, 162 points
1 Masie Davies (Exeter Chiefs) 18 caps, five points
2 Kelsey Jones (Sale Sharks) 58 caps, 40 points
3 Sisilia Tuipulotu (Harlequins) 35 caps, 50 points
4 Branwen Metcalfe (Brython Thunder) seven caps
5 Georgia Evans (Saracens) 49 caps, 15 points
6 Kate Williams (Gloucester-Hartpury co-captain) 27 caps, 25 points
7 Alex Callender (Harlequins co-captain) 48 caps, 50 points
8 Bryonie King (Gwalia Lightning) 18 caps
Replacements:
16 Molly Reardon (Gwalia Lightning) 17 caps, ten points
17 Gwenllian Pyrs (Sale Sharks) 57 caps, 20 points
18 Elan Jones (Brython Thunder) One cap
19 Gwen Crabb (Brython Thunder) 43 caps
20 Jorja Aiono (Gloucester-Hartpury) Six caps
21 Sian Jones (Gloucester-Hartpury) 13 caps
22 Jenna De Vera (Brython Thunder) Three caps
23 Nikita Prothero (Gwalia Lightning) two caps

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